Interferon Beta DNA Treatment Delivered with Ichor Technology Reduces Atherosclerosis Progression in Mice

DNA Delivery Device Succeeds in Producing Anti-inflammatory Protein That Reduces Signs of Atherosclerosis

NEW ORLEANS--()--Summary: Proprietary DNA delivery technology enables sustained Interferon-beta production from muscle cells reduces atherosclerosis progression in mouse model of heart disease

Research conducted at Cleveland Clinic and presented today at the American College of Cardiologys 58th Annual Scientific Session showed that a single administration of DNA encoding the anti-inflammatory protein interferon-beta (IFN-ß) using Ichor Medical Systems TriGrid Delivery System (TriGrid) reduced the development of plaque formation in a mouse model of atherosclerosis.

The sustained production of this protein reduced the atherosclerotic lesion size and tissue-damaging inflammation that characterize the blood vessels of these mice with atherosclerosis, said Marc S. Penn, M.D., Ph.D., principal investigator from Cleveland Clinic.

While preliminary, these results broaden the support for the use of the electroporation-based TriGrid as an enabling platform technology to provide continuous endogenous production of therapeutic proteins from a patients own muscle. Previous studies done in collaboration with Dr. Vince Tuohy's lab at Cleveland Clinic demonstrated that one-time delivery of the IFN-ß gene intramuscularly with the TriGrid in a mouse model of multiple sclerosis (MS), resulted in sustained production of IFN-ß and a similar reduction in the severity of clinical disease as compared to a separate group of mice dosed every other day with recombinant IFN-ß (Molecular Therapy 14(3): 416-422 (2006)). This study suggests that in vivo gene transfer of the IFN-ß gene may provide a convenient and viable alternative to constant life-long dosing of the recombinant form of IFN-ß for patients with MS.

Together, these data demonstrate both the potential therapeutic and economic value of Ichors technology.

The TriGrid platform makes treatments like interferon-ß more reasonable because a single administration of the gene using the TriGrid results in consistent production of the therapeutic protein in muscle for extended periods of time resulting in a vastly reduced cost, said Ichor CEO Bob Bernard.

The TriGrid is also being used to enable endogenous protein production for DNA-based treatments of HIV, Hepatitis B and C, malaria, avian flu and various forms of cancer in pre-clinical and clinical trials worldwide.

About Cleveland Clinic

Cleveland Clinic, located in Cleveland, Ohio, is a not-for-profit multispecialty academic medical center that integrates clinical and hospital care with research and education. Cleveland Clinic was founded in 1921 by four renowned physicians with a vision of providing outstanding patient care based upon the principles of cooperation, compassion and innovation. U.S. News & World Report consistently names Cleveland Clinic as one of the nations best hospitals in its annual Americas Best Hospitals survey. Approximately 1,800 full-time salaried physicians and researchers at Cleveland Clinic and Cleveland Clinic Florida represent more than 100 medical specialties and subspecialties. In 2005, there were 2.9 million outpatient visits to Cleveland Clinic. Patients came for treatment from every state and from more than 80 countries. There were nearly 54,000 hospital admissions to Cleveland Clinic in 2005. Cleveland Clinics Web site address is www.clevelandclinic.org.

About Ichor Medical Systems

Ichor Medical Systems, a privately-held biotech company, is developing products based on the in vivo application of electroporation to enhance intracellular delivery of DNA drugs encoding therapeutic proteins or antigens for vaccines. Ichors proprietary TriGrid Delivery System (TriGrid) is the first and only integrated and fully automated system for electroporation-mediated DNA administration. This system allows the site of DNA injection, placement of electrodes, rate of DNA administration and timing of electrical pulse application to be consistent among different operators and patients. As a result, Ichors TriGrid enables safe, effective and reproducible clinical application of electroporation in a manner capable of supporting development and commercialization of DNA-based products. The Ichor system is currently being used on three continents in a wide range of pre-clinical and clinical studies for potential treatments of melanoma, HIV, Hepatitis B, Avian Flu and Multiple Sclerosis.

Contacts

Gable PR: 877-251-3888
Erin Koch
Cell: 619-261-0762
erin@gablepr.com
Tom Gable
Cell: 619-251-3881
tom@gablepr.com

Contacts

Gable PR: 877-251-3888
Erin Koch
Cell: 619-261-0762
erin@gablepr.com
Tom Gable
Cell: 619-251-3881
tom@gablepr.com