New System from Baker Ruskinn Accelerates Oxygen Regulation in Culture Media

Protocol-driven, tested solution helps improve research results, enhance experimental control and increase productivity

SANFORD, Maine--()--HypoxyCOOLTM, the new media conditioning system from Baker Ruskinn, accelerates the reduction of oxygen concentration in tissue/cell culture media to user-defined values by as much as two days. The system is the first commercialized solution for precisely controlling oxygen levels in liquid media used in stem cell expansion or any tissue culture process requiring accurate and stable oxygen levels.

“Exposing cells native to low-oxygen environments to normal atmospheric conditions causes abnormal cell reactions and reduces cell viability,” said Science Director Deborah Thibodeaux. “Even when gas-controlled incubators or workstations are used to control the atmosphere around cultures, they could still remain susceptible to oxidative stress due to higher concentrations of dissolved oxygen within cell growth media.”

HypoxyCOOL combats this oxidative stress by precisely reducing media oxygen concentration to user-defined values – in as little as three hours.

A Sophisticated Alternative to Problematic Conditioning Methods

The typical process for conditioning (i.e., reducing the oxygen concentration in) cell culture media is imprecise, time-consuming and unpredictable. The result is higher-than-expected dissolved oxygen levels, which may skew research results due to cellular behavior reflective of adaptation to abnormal oxygen concentrations.

HypoxyCOOL solves these problems by enabling researchers to:

  • Enhance experimental control. Several variables that impact media conditioning are difficult to control using conventional processes. These variables include changes in media pH, potential microbial contamination, altitude, temperature and the presence of carbon dioxide. HypoxyCOOL helps control these variables and provides a repeatable conditioning protocol that improves the consistency of research results.
  • Increase productivity. Obtaining true, physiologically-relevant oxygen levels in media requires significant time – it may even take up to two days to achieve a dissolved oxygen concentration of 2% (see Figure 1). Moreover, this process must be repeated each time media conditioning is required. HypoxyCOOL accelerates the media conditioning process, allowing dissolved oxygen concentrations to reach 2% within as little as three hours. Additionally, oxygen levels stay low for up to 21 days, eliminating the need for reconditioning, significantly enhancing productivity in the lab.
  • Improve research results. In addition to the research conducted in Baker Ruskinn’s laboratory, independent research has shown that using in vivo-like oxygen levels in culture media stabilizes cell cultures, enhances gene expression and increases viability and transcriptional stability.

For more information about HypoxyCOOL, read our blog on Regulating Dissolved Oxygen Levels in Culture Media, or visit http://www.ruskinn.com/products/hypoxycool.

ABOUT BAKER RUSKINN

Baker Ruskinn, is a global leader and supplier of anaerobic and modified atmosphere solutions for microbiology and tissue/cell culture applications. Its advanced line of anaerobic chambers, hypoxia workstations and media conditioning solutions help improve research results by providing precisely controlled conditions for anoxic and low-oxygen studies. Baker Ruskinn’s most recent innovation, HypoxyCOOLTM, is the first commercialized system for quickly and precisely regulating dissolved oxygen levels in culture media, helping to improve research results, laboratory productivity and experimental control. To learn how Baker Ruskinn products can benefit your research, visit www.ruskinn.com.

Contacts

The Baker Company
Cheri Gaudet, 800-992-2537
Fax: 207-324-3869
www.bakerco.com

Figure 1: HypoxyCOOL reduces dissolved oxygen concentration in culture media within hours (vs. days with conventional conditioning methods) (Graphic: Business Wire)

Figure 1: HypoxyCOOL reduces dissolved oxygen concentration in culture media within hours (vs. days with conventional conditioning methods) (Graphic: Business Wire)

???news_view.multimedia.download???

???pagination.previous??? ???pagination.next???

Release Summary

HypoxyCOOL™ from Baker Ruskinn is the first commercialized system that accelerates the reduction of dissolved oxygen concentration in cell culture media to user-defined values by as much as two days.

Sharing

Contacts

The Baker Company
Cheri Gaudet, 800-992-2537
Fax: 207-324-3869
www.bakerco.com