Baylor College of Medicine to Use Applied Biosystems Genetic Analysis Technology as Part of 1000 Genomes Project

HOUSTON & FOSTER CITY, Calif.--(BUSINESS WIRE)--Scientists at the Baylor College of Medicine Human Genome Sequencing Center (HGSC) will use high-throughput sequencing systems from Applied Biosystems (NYSE:ABI), an Applera Corporation business, for a significant part of their contribution to the first pilot phases of the 1000 Genomes Project, sponsored by the National Human Genome Research Institute (NHGRI), the Wellcome Trust and the Beijing Genome Institute. This project is a worldwide research effort that will involve the sequencing of 1,000 genomes from people from around the world to create the most detailed and medically useful picture to date of human genetic variation. The HGSC will acquire six SOLiD™ Systems in order to complete the work.

The data generated as part of the 1000 Genomes Project is expected to reveal clues about how variant DNA sequences contribute to conditions such as cancer, diabetes and heart disease. The HGSC is utilizing the SOLiD System to expand its contribution of the first phases of the project and help researchers to determine the best methods for sequencing these 1,000 human genomes.

The first phases of the project began earlier this month. When the pilot phase of this project is complete, the HGSC will have used the SOLiD System to generate approximately 200 billion bases of sequence data over a span of four months. This sequence data will consist of significant sequence coverage of 24 human genome samples; and much deeper coverage of a single human genome sample. This amount of data is equivalent to the entire contents of GenBank, the largest public repository of DNA sequence data.

In the analysis of human genetic variation, the depth of coverage refers to the number of times each of the approximately 3 billion base-pairs of DNA from one genome is read by a genetic analysis system. Deeper coverage of a genome increases the confidence researchers have in characterizing the bases that exist at each position within a genome. As a result, researchers are better able to recognize the occurrence of variants in the genome. According to the HGSC, one goal of the pilot phase of the 1000 Genomes Project will be to determine the depth of sequence coverage from different data types that are needed in order to fully understand how sequences of DNA in the genome vary significantly between individuals.

One reason why the HGSC chose to use the SOLiD System for this project is because of its extremely high throughput capability. The SOLiD System has now demonstrated that it can produce greater than 10 gigabases per run, which is more than 3x genome coverage. The throughput of the SOLiD System establishes it as the highest throughput genetic analysis system available today. An ultra-high-throughput genetic analysis system will help enable scientists at the HGSC to complete this project in an efficient and cost-effective manner.

“There is clearly a role for very high density data from platforms that generate read lengths in the 25-50 base range,” said Dr. Richard Gibbs, director of the HGSC at Baylor College of Medicine. “We believe that the SOLiD System will dominate in this arena. The production and pooling of data from multiple sources and platforms on the same samples in the 1000 Genomes Project will help researchers ultimately determine the genetic analysis platform of choice.”

Although most human genetic information is the same in all people, researchers are generally more interested in studying the small percentage of genetic material that varies among individuals. Researchers characterize genetic variation as either single-base changes – single nucleotide polymorphisms – or as a series of larger stretches of sequence variation known as structural variants. To effectively characterize SNPs in the genome for the 1000 Genomes Project, researchers must be able to distinguish real genetic variants from sequencing errors, which requires a highly accurate genetic analysis system. A combination of depth of coverage and a highly accurate genetic analysis system helps researchers identify the genetic differences that exist between individuals.

Use of higher accuracy genetic analysis systems will require lower depth of coverage to confidently characterize variants in genome samples. HGSC’s decision to use the SOLiD System for the 1000 Genomes Project was in part based on a comparative study of microbial and mammalian genomes sequenced by the SOLiD System and competing short-read platforms.

“Accuracy is vital, not just for the 1000 Genomes Project, but for all other applications, too,” said Donna Muzny director of operations at the HGSC. “The internal error-checking strategy for the SOLiD System makes it superior for the read lengths that are produced.”

In deciphering the human genome, researchers strive to both accurately identify and locate genetic variants in the genome. Mate pair analysis, or the ability of a genetic analysis system to analyze pairs of sequences separated by a known distance between them – known as the insert size – allows researchers to determine the precise location of structural variants in the genome. Structural variants consist of gene copy number variations, single base duplications, inversions, translocations, insertions and deletions. For instance, by analyzing insert sizes up to 10,000 base pairs, the SOLiD System can cover sequences that span large regions of repeated patterns within the genome. Mate pair analysis also enables accurate placement of sequence reads in and around repeat regions, which can vary greatly among individuals. This capability of the SOLiD System will help researchers to accurately piece together large numbers of DNA sequence fragments as they assemble these sequences into entire human genomes.

Once researchers determine the depth of coverage necessary to confidently characterize SNPs and structural variants in human genome samples, they will be able to make better use of genomic information. For instance, researchers will be able to more effectively use this information to understand how these variations are related to an individual’s susceptibility to disease and response to treatment for disease, which is the promise of personalized medicine.

“The HGSC’s decision to scale up on the SOLiD System for a large population resequencing project further validates the benefits of the platform for these types of studies,” said Shaf Yousaf president for Applied Biosystems’ molecular and cell biology genomic analysis division. “The successful completion of this project should enable broader applications of genomic analysis and open the door for a host of personalized medicine, and disease association studies. We believe this project will move life-science researchers a step closer to defining the precise relationship between human genetic variation and disease.”

About the SOLiD System

The SOLiD System is an end-to-end next-generation genetic analysis solution comprised of the sequencing unit, chemistry, a computing cluster and data storage. The platform is based on sequencing by oligonucleotide ligation and detection. Unlike polymerase sequencing approaches, the SOLiD System utilizes a proprietary technology called stepwise ligation, which generates high-quality data for applications including: whole genome sequencing, chromatin immunoprecipitation (ChIP), microbial sequencing, digital karyotyping, medical sequencing, genotyping, gene expression, and small RNA discovery, among others.

Unparalleled throughput and scalability distinguish the SOLiD System from other genetic analysis sequencing platforms. The system can be scaled to support a higher density of sequence per slide through bead enrichment. Beads are an integral part of the SOLiD System’s open-slide format architecture, which enables the system to generate greater than 6 gigabases of sequence data per run. The SOLiD System has demonstrated runs greater than 10 gigabases per run at customer locations. The combination of the open-slide format, bead enrichment, and software algorithms provide the infrastructure for allowing it to scale to even higher throughput, without significant changes to the platform’s current hardware or software.

About Applera Corporation and Applied Biosystems

Applera Corporation consists of two operating groups. Applied Biosystems serves the life science industry and research community by developing and marketing instrument-based systems, consumables, software, and services. Customers use these tools to analyze nucleic acids (DNA and RNA), small molecules, and proteins to make scientific discoveries and develop new pharmaceuticals. Applied Biosystems’ products also serve the needs of some markets outside of life science research, which we refer to as “applied markets,” such as the fields of: human identity testing (forensic and paternity testing); biosecurity, which refers to products needed in response to the threat of biological terrorism and other malicious, accidental, and natural biological dangers; and quality and safety testing, such as testing required for food and pharmaceutical manufacturing. Applied Biosystems is headquartered in Foster City, CA, and reported sales of approximately $2.1 billion during fiscal 2007. The Celera Group is a diagnostics business delivering personalized disease management through a combination of products and services incorporating proprietary discoveries. Berkeley HeartLab, a subsidiary of Celera, offers services to predict cardiovascular disease risk and optimize patient management. Celera also commercializes a wide range of molecular diagnostic products through its strategic alliance with Abbott and has licensed other relevant diagnostic technologies developed to provide personalized disease management in cancer and liver diseases. Information about Applera Corporation, including reports and other information filed by the company with the Securities and Exchange Commission, is available at http://www.applera.com, or by telephoning 800.762.6923. Information about Applied Biosystems is available at http://www.appliedbiosystems.com. All information in this press release is as of the date of the release, and Applera does not undertake any duty to update this information unless required by law.

Applied Biosystems Forward Looking Statements

Certain statements in this press release are forward-looking. These may be identified by the use of forward-looking words or phrases such as "should, "planned," and "expect," among others. These forward-looking statements are based on Applera Corporation's current expectations. The Private Securities Litigation Reform Act of 1995 provides a "safe harbor" for such forward-looking statements. In order to comply with the terms of the safe harbor, Applera Corporation notes that a variety of factors could cause actual results and experience to differ materially from the anticipated results or other expectations expressed in such forward-looking statements. These factors include but are not limited to: (1) rapidly changing technology and dependence on customer acceptance of the SOLiD System; (2) the risk of unanticipated difficulties associated with the further development of the SOLiD™ System; and (3) other factors that might be described from time to time in Applera Corporation's filings with the Securities and Exchange Commission. All information in this press release is as of the date of the release, and Applera does not undertake any duty to update this information, including any forward-looking statements, unless required by law.

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